Diversity of gut microbiota in Locusta migratoria manilensis Meyen from different regions of China

Abstract:

[Objective] Gut microbiota plays an important role in the environmental adaptation of insects. This study aims to explore the diversity of gut microbiota in Locusta migratoria manilensis Meyen from different regions of China. [Methods] The Illumina NovaSeq platform was used to sequence the 16S rRna gene of gut microbiota in L. migratoria manilensis Meyen from four representative locust regions in China. [Results] Geographical location, development stage, and gender affected the structure of gut microbiota, among which geographical location had the most significant effect, followed by gender and development stage. The L. migratoria manilensis Meyen from Qingyuan City, Guangdong Province and Cangzhou City, Hebei Province showed the largest difference in the diversity of gut microbiota. Mean temperature and precipitation among the eight selected environmental factors may influence the gut microbiota diversity. [Conclusion] This study analyzed the environmental adaptability of L. migratoria manilensis Meyen from the perspective of symbiotic microorganisms and environmental factors, which provided a basis for the development of technology for tracing geographical origin and microbial preparations.