Phage therapy for the mouse pulmonary infection caused by Pseudomonas aeruginosa

Abstract:

[Objective]A murine pneumonia model infected by Pseudomonas aeruginosa was established and treated with a phage cocktail in two ways,and then the therapeutic effect was evaluated.[Methods] We injected 50 μL P.aeruginosa suspension of 2×10⁸ CFU/mL into the trachea after performing the tracheostomy in mice.After half an hour,a phage cocktail preparation was administrated into the infected mice through intranasal and intraperitoneal routes.At the same time,we established two control groups with phosphate-buffered saline in parallel.We then observed the physiological state of the mice and examined the bacteriological,pathological,and inflammatory levels 24 h post infection.[Results]P.aeruginosa was eliminated in the mice administrated with the phage cocktail preparation.The mice treated the phage cocktail showed intact lung tissue structure and slight inflammation compared with the control group.[Conclusion]The phage cocktail preparation can inhibit P.aeruginosa in mice and has a certain therapeutic effect on bacterial infections in the lungs.