Metagenomic next-generation sequencing in diagnosis of infectious diseases of central nervous system

Abstract:

Infections of central nervous system (CNS) are acute or chronic inflammatory (or non-inflammatory) diseases caused by the invasion of viruses,bacteria,or fungi in CNS.Infectious diseases of CNS have high mortality rate with serious sequelae.Due to the limitation of detection strategies and low sensitivity,the pathogens of more than half of patients with infectious diseases of CNS cannot be identified by traditional methods.Metagenomic next-generation sequencing (mNGS) is a new technology and can significantly improve the detection rate of pathogens.However,mNGS is still not well understood by a few clinicians and other related people,which limits its rapid promotion and application in clinical diagnosis and treatment.This review systematically introduced the whole process of mNGS,and summarized the development history and the latest research progress of mNGS in the diagnosis of infectious diseases of CNS,thereby providing references for the diagnosis and treatment of infectious diseases of CNS.