Discovery and characterization of a PKS-NRPS hybrid in Arthrinium arundinis ZSDS1-F3 by genome mining

Abstract:

[Objective] To activate the biosynthesis gene cluster for polyketide synthases-nonribosomal peptide synthetases (PKS-NRPS) from the marine-derived Arthrinium arundinis ZSDS1-F3 based on genome analysis, and to isolate and identify the secondary metabolites. [Methods] The cryptic biosynthesis gene cluster was activated by promoter engineering and heterologous expression. The products were isolated and elucidated by HR-ESI-MS and 1D and 2D NMR, and the biosynthetic pathways of them were deduced based on gene recombination and bioinformatics analysis. [Results] A dormant PKS-NRPS-encoding gene cluster from A. arundinis ZSDS1-F3 was activated through heterologous expression in Aspergillus nidulans A1145. Two new products were isolated and the biosynthetic pathways were clarified. [Conclusion] Via bioinformatics analysis, genome mining plays an important role in the discovery of novel natural products from fungi.