Isolation and identification of microorganisms in Nitraria tangutorum Bobr. rhizosphere in Lanzhou

Abstract:

[Objective] Microorganisms with potential growth-promoting effects on plants were isolated from the rhizosphere of Nitraria tangutorum Bobr. in Lanzhou City, which laid a foundation for research and development of related preparations.[Methods] With the plate streak method, 6 bacterial strains were isolated and purified from the rhizosphere of N.tangutorum, and the morphological characteristics of the strains were observed. The physiological and biochemical properties were examined based on Gram staining and other tests. The effect of each strain on quinoa growth was determined. The 16S rRNA gene of a strain with excellent growth-promoting effect was sequenced and the circular genome map for the strain was plotted.[Results] According to the biochemical identification results, the 6 strains belonged to Acinetobacter, Agrobacterium, Paenibacillus, Bacillus, Sphingomonas, and Pseudomonas, respectively. Further identification by 16S rRNA gene sequencing suggested that BC4, with strong growth-promoting activity, belonged to Enterobacter.[Conclusion] BC4 shows strong growth-promoting activity, which lays a theoretical basis for development and utilization of bacterial resources of N.tangutorum in Lanzhou.