The effect of LytSR and PdtaSR two-component systems on the biosynthesis of butenyl-spinosyn of Saccharopolyspora pogona

Abstract:

[Objective] There are some two-component systems in the genome of Saccharopolyspora pogona, including a LytR regulatory factor and a PdtaR transcription resistance termination regulator, which were analyzed through genetic engineering technology.[Methods] In this study, we performed the fusion PCR and inter-genus conjugative transfer technology to construct S. pogona-∆lytR and S. pogona-PdtaR, and studied their effects on the biosynthesis of butenyl-spinosyn.[Results] We found that the production of butenyl-spinosyn in S. pogona-∆lytR was increased compared that in the wild type, while the butenyl-spinosyn production of S. pogona-PdtaR was dropt through high performance liquid chromatography (HPLC) experiments, indicating that lytR and pdtaR negetively regulating the biosynthesis of butenyl-spinosyn. In addition, we found that S. pogona-∆lytR and S. pogona-PdtaR also had certain impacts on the growth and sporulation.[Conclusion] This stydy laid a theoretical foundation for further elucidating the regulatory mechanism of butenyl-spinosyn biosynthesis.