Abstract:The human gastrointestinal tract harbors trillions of microorganisms which affect the host health. Probiotics, as a group of live microorganisms, play a role in maintaining the gut mucosal barrier function, regulating immune function, and promoting the metabolism and absorption of nutrients. Probiotics have demonstrated good performance in the treatment of gut microbiome disorders and gastrointestinal diseases such as functional dyspepsia, gastroenteritis, diarrhea, constipation, colic, irritable bowel syndrome, inflammatory bowel disease, and Helicobacter pylori infection. This article briefly overviewed the effects of probiotics on gastrointestinal health, aiming to provide support for consumers and professionals to understand their role in gastrointestinal health and use probiotics in daily health management.