Effect of long non-coding RNA CTO-S on the replication of pseudorabies virus

Abstract:

[Objective] This study aims to reveal the effect of the long non-coding RNA (lncRNA) CTO-S on the replication of pseudorabies virus (PRV). [Methods] We employed 3'RACE and 5'RACE to identify the full-length sequence of CTO-S, and verified whether CTO-S had the ability to encode polypeptides. After constructing the CTO-S deletion and complementation strains with the Red recombination method, we analyzed the differences in the replication of wild-type, deletion, and complementation strains. We used RNA pull down combined with mass spectrometry to screen out the host protein interacting with CTO-S, and then adopted RNA immunoprecipitation (RIP) method to verify the interaction between the screened protein and CTO-S. Finally, the methyl tetrazolium (MTT) method was used to detect the effects of wild-type, deletion, and complementation strains on cell apoptosis. [Results] CTO-S had a full length of 267 bp and did not have the ability to encode polypeptides. The deletion of CTO-S did not affect the replication of PRV. CTO-S interacted with cytochrome b-c1 complex subunit 1, a component of ubiquinol-cytochrome c reductase, and induced apoptosis. [Conclusion] CTO-S is not essential for the replication of PRV. It interacts with cytochrome b-c1 complex subunit 1, a component of ubiquinol-cytochrome c reductase, and induces cell apoptosis.