Kinetics of cell division cyclin protein Cdc5 in budding yeast during mitosis at different temperatures

Abstract:

[Objective] To explore the molecular dynamics changes of cell division cyclin protein Cdc5 in budding yeast during mitosis at different temperatures.[Methods] In this study, Saccharomyces cerevisiae was used as the experimental material objective to explore the molecular dynamic changes of Cdc5 protein in the mitosis process of budding yeast at different temperatures by using living cell imaging method; draw the growth curve by measuring OD₅₉₅ to see whether the macro division is consistent with the micro dynamic changes of Cdc5 protein; use flow cytometry to detect the cell cycle changes.[Results] During cytokinesis, Cdc5 protein entered into daughter cells from mother cells and aggregated at bud neck. The aggregation time of Cdc5 protein at the bud neck was long at 25℃, and the aggregation time of Cdc5 protein at the bud neck was short at 37℃. There was a significant difference between them. However, there was no significant difference in the expression of Cdc5 protein between the two temperatures. At the same time, the temperature also affected the dynamics of Cdc5 protein during degradation process, including the occurence of frequency and time of the peak of fluorescence intensity of Cdc5 in mother cells and daughter cells. The growth curve results showed that the single cell division cycle of budding yeast affected its macroscopic cell growth, and the faster the division rate of budding yeast, the smaller the ratio of length to width of the daughter cell. The cell cycle results showed that the dynamic changes of Cdc5 protein at 37℃ were consistent with the cell cycle changes of budding yeast. The cell cycle results showed that the cell cycle of budding yeast changed from G₀/G₁ phase to S phase at 37℃, which also accelerated the division of budding yeast.[Conclusion]] This study was to explore the molecular dynamics of Cdc5 protein in the mitosis of budding yeast at different temperatures and the corresponding macro growth of budding yeast for the first time. The results showed that the temperature had an effect on the dynamics of Cdc5 protein, and its molecular dynamics was positively correlated with the division speed of budding yeast, which provided a basis for further study of its function in mitosis.