Fast-preparation of compost effective microorganisms (EM) for perishable garbage based Environmental Microbial Repository

Abstract:

[Objective] Fast-preparation of effective microorganisms for perishable garbage compost.[Methods] Based on the Environmental Microbial Repository supported by the "Biological Resources Service Network Initiative", we selected excellent organic matters decomposition strains (protein, starch, cellulose, lignin, oil) according to microbial enzyme activity and perishable organic matters. The compost effective microorganisms (CM) were prepared according the antagonism between microbes. Subsequently, we studied the optimal fermentation condition of CM and the perishable garbage compost.[Results] The optimal fermentation condition for CM was inoculating 7% seed liquid in brown sugar medium and culturing for 48 h at 30℃. At this time, the activities of protease, amylase, cellulase, ligninase, and lipase in the fermentation solution were 181.76, 52.92, 1.57, 12.81, 9.94 U/mL, respectively. The pH, moisture content, and organic content of CM compost product meet the bioorganic fertilizer standard (NY 884-2012). The composting temperature of the CM group could reach 63.5℃ and maintain at the high temperature for 9-12 d, at the end of composting, the C/N, moisture content and organic matter degradation rate of CM compost product were 8.93, 28.7%, 30.4%. Compared with the control group, CM could accelerate the degradation of organic matters, and has a stronger nitrogen fixation capacity, shorten the decay time, improve the quality of compost products.[Conclusion]] It is a fast and effective method to prepare the effective microorganisms based on the Environmental Microbial Repository.