Identification of an methylarsenite demethylation bacterium Bacillus aryabhattai FJ-6 and characterization of methylarsenite demethylation gene

Abstract:

[Objective] The purpose of this study is to isolate and identify the MAs(III) demethylation strains. We cloned and expressed the arsI gene involved in MAs(III)-demethylation in Bacillus aryabhattai FJ-6 and characterized the ArsI protein.[Methods] The arsI gene fragment (432 bp) was amplified with PCR. Recombinant plasmid pET29a-arsI was constructed and transformed into Escherichia coli BL21(DE3) for heterologous expression, and the expressed protein was detected with SDS-PAGE. The enzyme activity of ArsI was determined by using HPLC-ICP-MS.[Results] The arsI gene was cloned and the recombinant plasmid pET29a-arsI was expressed. The molecular weight of the recombinant protein was 17.4 kDa.[Conclusion]] The activity of ArsI protein with MAs(III) demethylation was clarified.