Advances in the detection of pathogenic bacteria by using loop-mediated isothermal amplification-based microfluidic chip

Abstract:

Loop-mediated isothermal amplification (LAMP) is an emerging nucleic acid amplification technique under isothermal condition, which can combined with microfluidic chip to rapidly detect the pathogenic bacteria. It is of higher specificity, higher sensitivity and simpler operation. This article classified and introduced the LAMP-based microfluidic chip for the detection of pathogenic bacteria, based on the analysis of different end-point detection methods. And also, we analyzed the improvement and existing problems of this technology, which can provide great reference value for the further studies and industry development.