Perspectives and applications of CRISPR/Cas-mediated genome editing in lactic acid bacteria

Abstract:

Lactic acid bacteria are an important class of food industry microorganisms. At present, the identification and excavation of functional genes of lactic acid bacteria mainly rely on traditional gene homologous recombination technology. Although this technology has high reliability, there are complicated operations and low efficiency, which severely restrict the genetic selection of lactic acid bacteria. CRISPR/Cas gene editing technology has greatly improved the editing efficiency of multi-species genomes, which provides opportunities for rapid functional gene identification and genetic improvement of lactic acid bacteria. However, the application of existing CRISPR/Cas gene editing technology in lactic acid bacteria still has many limitations. This article summarizes the application status of CRISPR/Cas gene editing technology in the genome of lactic acid bacteria and the urgent problems to be solved, and prospects the future development of the lactic acid bacteria genome editing technology, providing references for the identification and genetic improvement of lactic acid bacteria.