Diversity of culturable yeasts in Yamzhog Yumco Lake

Abstract:

[Objective] To investigate culturable yeast diversity in Yamzhog Yumco Lake and analyze the influence of environmental factors on yeast diversity. [Methods] Yeasts were isolated by membrane filtration flat culture. Identification was based on sequence analysis of ITS domains of rRNA gene, combining with traditional classification method. SPSS 20.0 and CANOCO 5 were used to examine correlations between culturable yeast diversity and environmental factors. [Results] In total of 297 yeast strains were isolated, these strains were identified as 25 species in 16 genera. Vishniacozyma was the dominating genera in the Lake. Vishniacozyma victoriae was the dominating species. Statistical analysis indicated that pH, electric conductivity (EC), total dissolved salt (TDS) and salt were significantly positive related to yeast species number and genera number among different sites, total phosphorus (TP) is significantly negative related to yeast genera number among different sites and different regions, TP is significantly negative related to yeast species number among different regions, additionally, pH and TP were important factors influencing the community structure of yeast in the Yamzhog Yumco Lake. [Conclusion] The yeast community from Yamzhog Yumco Lake showed high species richness, the yeast community structure was different at different sites, and anthropogenical influenced is an important factor for the yeast community. The relationship between yeast community and human activities deserves further study.