Abstract:The resource utilization and harmless disposal of tobacco waste require efficient microbial degradation of refractory substances such as lignin and nicotine. This paper reviews the progress in biodegradation of refractory substances in tobacco waste. Up to now, an increasing number of strains capable of degrading lignin and nicotine have been isolated, and there are many publications on the mechanism and application of lignin and nicotine microbial degradation. However, there are few publications about their application in tobacco waste treatment. Lignin and nicotine-degrading bacteria also exhibit potential for lignin abatement and nicotine removal of waste tobacco leaves (tobacco stems). However, it is necessary to evaluate both the degradation ability and the adaptability to the real environment. Strains capable of degrading both lignin and nicotine are preferable for application, but few such strains have been isolated. The research of complex microflora based on complete genome analysis and microbiome technology, is obligated and will promote the development and application of microbial disposal technology for waste tobacco leaves containing lignin, nicotine and other refractory substances.