Effect of interaction between arbuscular mycorrhizal fungi and rhizosphere bacteria in farmland soils on nutrients utilization

Abstract:

Soil microbes are important drivers of nutrient cycling in soil-crop system. Arbuscular mycorrhizal fungal (AMF) communities are reported to promote plant nitrogen and phosphorus utilization, especially under adversity stress. [Objective] The aim of this study is to explore the cross-kingdom microbial network between AMF and rhizosphere bacterial communities, and disentangle the potential keystones related to nutrient utilization. [Methods] Illumina sequencing and CoNet were used to construct the microbial network, and partial redundancy analysis and correlation analysis was used to explore potential keystones. [Results] Positive associations were dominant between AMF and rhizosphere bacteria in the three soil types. The interactions between AMF and bacterial communities varied in the three soils types. Cross-kingdom interactions were highest in Acrisol, mainly between Glomus and Actinobacteria and Proteobacteria. And in Phaeozem the interactions were mainly intra-kingdom interactions among rhizosphere bacteria. The microbial taxa that significantly correlated with nutrient utilization were species from Glomus, Actinobacteria and Alphaproteobacteria. [Conclusion] The positive interrelationship between AMF and rhizosphere bacteria in typical dryland soil has a potential promotion effect on the utilization of nitrogen and phosphorus. The role of keystones in Acrisol with relatively low organic matters and nutrient may be more important.