Abstract:[Objective] To explore the effect of Clostridium butyricum on pathogenicity and protection mechanism of Salmonella enteritidis (CVCC3377) in SPF mice. [Methods] Mice were sacrificed on the 6th day post infection and samples of liver, spleen and cecum samples were collected. The pathological changes of liver and spleen tissue were detected by H.E staining, the expression levels of TLR4 and MyD88 protein in spleen tissue were detected by immunohistochemistry, and the expression levels of TNF-α and IL-6 in cecum tissue were detected by fluorescence quantitative PCR. The effect on intestinal flora was analyzed by high-throughput sequencing. [Results] C. butyricum could alleviate the weight loss of mice caused by Salmonella enteritidis infection. The results of HE staining show that Clostridium butyricum could alleviate the tissue damaged by Salmonella enteritidis infection. The results of immunohistochemistry show that Clostridium butyricum could decrease the expression levels of TLR4 and MyD88 protein in liver and spleen tissue cells. Fluorescence quantitative PCR shows that Salmonella enteritidis could significantly increase TNF-α in cecal tissue (P<0.05). The expression of TNF-alpha and IL-6 decreased by feeding Clostridium butyricum. The results of high throughput sequencing show that the Chao 1 index, ACE index and Shannon index of Clostridium butyricum treatment group were higher than those of the control group and Salmonella infection group (P>0.05). Clostridium butyricum could significantly reduce the proportion of Actinobacteria and Verrucomicrobia in intestinal microorganisms (P<0.05). In addition, the relative abundance of Lactobacillus in intestinal microbial community increased by bacteria (P>0.05). [Conclusion] Clostridium butyricum could improve the immunity of mice, alleviate the inflammatory reaction caused by Salmonella infection, increase intestinal microflora diversity and regulate intestinal flora structure.
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