Study on polycyclic tetramate macrolactam metabolites from mangrove-derived Streptomyces avicenniae

Abstract:

[Objective] Based on genome mining, we analyzed the secondary metabolites from mangrove-derived Streptomyces avicenniae 9-9, targeting on polycyclic tetramate macrolactams (PTMs). [Methods] Bioinformatics analysis was carried out to analyze the genome sequence of Streptomyces avicenniae 9-9. We searched PTMs by the method of high-performance liquid chromatography and mass spectroscopy. [Results] We found four genes, named aviA-D involved in PTMs biosynthetic gene clusters in Streptomyces avicenniae 9-9. Two PTMs, 1 and 2, were identified as ikaruganmycin and capsimycin B, respectively. Compound 3 through 5 also belonged to PTMs family, and their structures were proposed based on MS/MS fragmentation pattern. [Conclusion] Mangrove-derived Streptomyces avicenniae 9-9 can produce PTMs compounds with the 5-6-5 fused tricyclic skeleton.