Fungal composition and diversity of tobacco phyllosphere from cured tobacco leaves

Abstract:

[Objective] To know the fungal community composition and diversity of tobacco phyllosphere with different degrees of tobacco pole rot. [Methods] The fungal community composition of both petiole and leaf samples from flue-cured tobacco leaves was analyzed based on Illumina MiSeq high-throughput sequencing platform. [Results] The fungi from severe infected leaf samples (BQ), severe infected petiole samples (BZ), light infected leaf samples (JQ) and light infected petiole samples (JZ), were distributed in 7 phyla (Ascomycota, Basidiomycota, Zygomycota), 27 classes, 58 orders, 104 families, 171 genera and 360 fungal taxa (OTUs). The fungal community composition, relative abundance and dominant fungal taxon of each sample with different pole rot level were all different. When at genus level for sample BQ, the dominant genus was Aspergillus (89.64%), Myrothecium (2.54%), Rhodotorula (2.48%), Gibberella (1.49%). For sample BZ, dominant genus was Aspergillus (96.93%) and Alternaria (1.92%). For sample JQ, dominant genus was Aspergillus (40.13%), Rhodotorula (31.81%), Alternaria (16.75%). For sample JZ, the dominant genus was Aspergillus (62.77%), Alternaria (9.74%), Rhodotorula (5.20%). The diversity of fungal communities is the highest for sample JQ, richness of fungal communities is the highest for sample JZ. The richness and diversity of BQ fungal community were higher than those of BZ. Tobacco pole rot is a common disease in curing barn. This pathogenic fungus is various and widely distributed in tobacco leaf and environment. [Conclusion] The results provided a reference for the prevention and control of tobacco pole rot in flue-cured barn according to the characteristics of fungal community composition in different parts of the disease severity.