Screening of genes induced by exogenous phenolic on mycorrhized Poncirus trifoliate

Abstract:

[Objective] It has been reported that phnolics contents could be changed by the inoculation with arbuscular mycorrhizal (AM) fungi. In the present study, the differentially expressed genes were screened, and the genes function was analyzed with the exogenous phenolic addition. [Methods] Poncitrus trifoliata (L.) inoculated with AM fungi was treated by exogenous phenolic. We obtained the differentially expressed bands by differential-display RT-PCR (DDRT-PCR), and then cloned the related genes and analyzed the bioninformation. [Results] A total of 154 differentially accumulated transcript-derived fragments were identified. After the sequencing and blast of 16 fragments, 9 genes were found related to putative functions which participated into the recognition of environment factors and internal signals, and the adjust of mycorrhizal formation. The bioinformatic analysis showed that the cDNA sequences of DD-11 was 1382 bp in length, containing a complete 1080 bp open reading frame and 454 amino acids were encoded, which was only expressed under mycorrhizal inoculation and exogenous phenolic. The molecular weight, theoretical pI of the DD-11 gene deduced protein were 49950.03 Da, 7.81, C₂₂₁₀H₃₄₂₇N₆₀₃O₆₅₇S₃₁. The deduced DD-11 protein included 22 Ser, 14 Thr, 7 Tyr, which became the phosphorylation sites of protein kinase. The secondary structure of the deduced DD-11 protein mainly included Alpha helix, Random coil, Extended strand, Beta turn, which were 21.81%, 57.71%, 20.48% and have no Beta turn. [Conclusion] The genes related to phenolic expressed in mycorrhizal formation were obtained in our study, which were related closely to the plants signal recognition. Our study provide a new perspective of molecular mechanism of phnolics occurring in mycorrhizal formation.