Effects of banana pseudostem biochar on bacterial abundance and community structure in rhizosphere soil

Abstract:

[Objective] To study the effect of banana pseudostem biochar on soil microorganisms in banana rhizosphere, we added the biochar into soil. [Methods] We mixed banana pseudostem biochar (BPB) with soil uniformly at the mass ratio of 0, 1%, 2% and 3%. Banana rhizosphere soil was separated after 3 months of pot culture. The bacterial community structure and abundance in rhizosphere soil were characterized by 16S rRNA high throughput sequencing. [Results] Increasing BPB application could increase soil organic matter, available potassium, available phosphorus contents and increase soil pH value, but decrease available nitrogen concentration. In total 2278 OTUs were obtained from 1% BPB samples, showing the greatest diversity of bacterial communities. When 3% BPB was applied to the soil, the relative abundance of Bacteroidates, Verrucomicrobia and Firmicutes increased significantly, whereas that of Actinobacteria, Acidobacteria and Gemmatimonade decreased significantly. Principal component analysis showed that soil bacterial communities were similar between 1% BPB and 2% BPB treated samples. [Conclusion] We changed the bacterial abundance and community structure in rhizosphere soil by applying different proportion of BPB, and the change in high proportion of BPB was more obvious.