Exploration of functional genes related to iron carrier secretion in the plant growth-promoting rhizobacteria Bacillus mycoides Gnyt1

Abstract:

[Objective] For the excavation of plant growth promoting rhizobacteria (PGPR) strains, the functional genes were analyzed and mapped in order to find out their scientific value and industrialization development and serve agricultural production. [Methods] Bacillus mycoides Gnyt1 strain was used as the material, and the second and third generation sequencing technology were combined to carry out the whole genome sequencing of the strain. The possible functional genes of the nuclear genome of the strain were analyzed, and genes secreting iron carrier-related strain were analyzed. [Results] The total length of the genomic sequence after splicing was 5597907 bp. The percentage of GC was 35.57%. There were 9 genes involved in the secretion of siderophore in this strain, of which 2 genes mainly existed in the Porphyrin metabolism pathway and were associated with intracellular iron transport metabolism related. [Conclusion] Through whole genome sequencing, the functional genes related to the secretion of siderophores were identified as GYT1 and GYT2, which makes it possible for verifying the gene functions.