Complete genome sequence of Marinomonas sp. FW-1, an arylsulfatase-producing bacterium isolated from red seaweed

Abstract:

[Objective] Marinomonas sp. FW-1 secretes high-activity arylsulfatase. This study was carried out to clone all the possible arylsulfatase in Marinomonas sp. FW-1 and investigate the motif information essential for arylsulfatase. [Methods] We used high-throughput sequencing to obtain the entire genome sequence of Marinomonas sp. FW-1. We carried out gene assembly, gene prediction, functional annotation and Clusters of Orthologous Groups clustering analysis. Thereafter, we cloned arylsulfatase genes in Escherichia coli and analyzed the activity of the recombinant proteins. [Results] The complete genome is of 3964876 bp in length, with an average Guanine and Cytosine content of 44.03%, encoding 3590 protein genes, containing 78 tRNA and 25 rRNA operons. Genome annotation indicated the presence of four possible arylsufatase genes. Among the four candidate genes, three genes contained C-X-P-X-R motif and their recombinant products were found to exhibit arylsulfatase activities. [Conclusion] This study revealed multiple arylsulfatase gene sequences in the complete genome Marinomonas sp. FW-1 strain, which predicted various potential applications of this strain. Moreover, C-X-P-X-R motif was confirmed to be essential for arylsulfatase activity.