Using cell sorting techniques to explore the recovery of individual Cryptococcus neoformans cells

Abstract:

[Objective] A method of sorting single cells of Cryptococcus neoformans by flow cytometry was established to determine factors influencing proliferation of single C. neoformans cells.[Methods] The Moflo XDP flow cytometric sorter was used. The clonal recovery rate of C. neoformans cell was determined under various conditions.[Results] Single C. neoformans cells sorting process by cytometry was established. Single C. neoformans cells could recover growth which was affected by nutritional conditions and strains. Under nutrient-rich conditions, the clonal recovery rates of C. neoformans stains JEC21 and H99 were 74% and 88%, respectively. Under oligotrophic conditions, the clonal recovery rate of JEC21 was 37%; while the clonal recovery rate of H99 was 80%. The clonal recovery rate of JEC21 increased with increasing initial number of cells. When the cell number of JEC21 strain was 100, the clonal recovery rate was 55%; when the cell number was 1000, the clonal recovery rate was 97%.[Conclusion] The single C. neofroman cells sorted by flow cytometry had the capacity of recovery to growth, and the capacity was affected by nutritional conditions and strains.