Diversity and antibacterial activity of culturable actinobacteria from Xiaoerkule Lake

Abstract:

[Objective] The study is aimed to reveal the diversity of culturable actinobacteria in Xiaoerkule salt lake, to lay a foundation for further development. [Methods] Actinobacterial diversity of Xiaoerkule lake was studied by culturable method and phylogenetic analysis based on 16S rRNA gene sequences. Component factors of samples were tested by conventional methods. Activity for protease, amylase and asterase was tested by inoculating single colony method. Antibacterial activity of novel actinomycetes was detected by bacteriostasis ring method. [Results] In total 51 OTUs isolated from the salt lake belonged to 24 different genera, and 15 strains are novel actinomycetes. Streptomyces is predominated microorganisms for 16.25% of the total isolated amounts. Sample environmental factors affected the population of actinomycetes in this lake. Tested actinomycetes showed excellent functional enzyme activity and antibacterial activity. Strain XHU 5011, a novel actinomycete, showed great potential for anti-Staphylococcus aureus, anti-Mycobacterium smegmatis and anti-Pseudomonas fluoressens. [Conclusion] There is abundant actinobacterial diversity in the sediment of Xiaoerkule lake, and the result implies that there are large numbers of unknown actionobacterial groups here. These actinomycetes have excellent functional enzymes and provide the basis for further study on secondary metabolites.