Annexin-1 promotes type I interferon production and inhibits foot-and-mouth disease virus replication

Abstract:

[Objective] We studied the regulatory effect of Annexin-1(ANXA1) on type I interferon (I-IFN) production and foot-and-mouth disease virus (FMDV) replication.[Methods] We used overexpression and knockdown assays to determine the role of ANXA1 in FMDV-infected cells.Then we evaluated the influence of ANXA1 on ISRE and IFN-β promoter activation by dual luciferase reporter assays.To confirm the regulatory role of ANXA1 on type I IFN pathway,IFN regulatory factor 3(IRF3) phosphorylation was detected by Western blotting.Expression of interferon-stimulated genes (ISGs) was measured by RT-PCR to investigate the effect of ANXA1 on ISGs expression.[Results] Overexpression of ANXA1 significantly suppressed FMDV replication and knockdown of ANXA1 expression enhanced virus replication,showing an antiviral role of ANXA1 in FMDV-infected cells (P<0.01 or P<0.05).ANXA1 promoted the activation of type I IFN pathway in a dose-dependent manner.Overexpression of ANXA1 enhanced IRF3 phosphorylation and ISGs expression (P<0.01 or P<0.05).[Conclusion] ANXA1 enhances type I IFN production and suppresses FMDV replication.