Fusion function of Peste des petits ruminants virus envelope proteins

Abstract:

[Objective] To study the role of Peste des petits ruminants virus envelope glycoproteins hemagglutinin and fusion during the fusion process. [Methods] The eukaryotic expression recombinant plasmids pCMV-HA-H, pCAGGS-Flag-F, pCMV-Myc-SLAM and pCMV-Myc-Nectin 4 sequenced correctly were transfected or co-transfected into CHO-K1 cells, and then the fusion role of PPRV H and F proteins was analyzed by microscopy and indirect immunofluorescence technique. [Results] The formation of syncytium was observed in the co-transfected cells while it was not found in the single plasmid transfected cells and the negative control. The number of syncytia in cells co-expressing H and F proteins was significantly more than that of the other groups. The H and F proteins were co-redistributed to form the polar caps (co-capping) in the same position. [Conclusion] PPRV F protein is an essential protein for fusion of the viral membrane with host cell membrane, but it must interact with PPRV H to make virus invasion successfully.