Physiological and transcriptional responses of Actinobacillus succinogenes to acid stress

Abstract:

[Objective] We explored by physiological and transcriptional responses of Actinobacillus succinogenes CGMCC1593 to acid stress. [Methods] We studied the effects of different initial pH on cell growth, activity of H⁺-ATPase and intracellular pH before and after acid stress as well as the protection mechanism of glutamate on CGMCC1593. The transcriptomics technique was used to explore the differentially expressed genes under acid stress. [Results] Cell growth was inhibited and the activity of H⁺-ATPase decreased with the decrease of initial pH. After acid stress at pH 4.7, the cell membrane was severely damaged. Glutamate had a protective effect on the cells under acid stress. In total 39 genes were differently expressed altogether under acid stress, of which 49% were stress and transport proteins, and a small part was related to metabolism. [Conclusion] Understanding the physiological and transcriptional responses of Actinobacillus succinogenes to acid stress provides references for improving acid resistance of Actinobacillus succinogenes.