Chicken manure co-conversion by Hermetia illucens larvae with non-associated microorganism

Abstract:

[Objective] Aimed at studying co-conversion of chicken manure by black soldier fly larvae (BSFL) with non-associated microorganism, we evaluated the influence of larval non-associated microorganism on this conversion. [Methods] Bacteria were determined by plating serial dilutions of chicken manure and pig manure composting on LB agar plates. Each isolated strain was inoculated into sterile chicken manure and converted with BSFL. The conversion effect and the influence to BSFL were evaluated. Bacteria that efficiently promoted the conversion of chicken manure were selected and mixed in different proportions. Different bacteria mixtures were inoculated into fresh chicken manure with BSFL to analyze the efficacy of the mixed inoculation on manure conversion. [Results] Compared with the control, strain R-07, R-09, F-03 and F-06 significantly promoted the growth of BSFL and degradation of chicken manure. Conversion rate of the group inoculated with these four strains increased by 27.21%, 15.00%, 9.93% and 16.29%, respectively. Manure degradation rate increased by 17.94%, 10.42%, 7.84% and 9.27%, respectively. When the proportion of inoculated mixture was R-07:R-09:F-03:F-06=4:1:1:1, the co-conversion efficacy was the best. Compared with the control, the survival rate of BSFL increased by 10.25%, the BSFL weight increased by 28.41%, the conversion rate increased by 30.46%, and the manure degradation rate increased by 7.69%. [Conclusion] Chicken manure co-conversion by BSFL and selected non-associated microorganism could obviously enhance the efficacy of larval conversion. These results will help improve the BSFL conversion system and develop new-type co-conversion of livestock and poultry manure more efficiently.