Genomic analysis reveals the biosynthesis pathways of diverse secondary metabolites and pinoresinol and its glycoside derivatives in Phomopsis sp.XP-8

Abstract:

[Objective] Sequencing and analysis of Phomopsis sp. XP-8 genome are beneficial to reveal the potential metabolic pathways of this strain and the key genes related to the biosynthesis of pinoresinol and its glycoside derivatives and other secondary metabolites. [Methods] We sequenced Phomopsis sp. XP-8 genome by the Illumina HiSeq 2500 high throughput sequencing platform. Then gene prediction and functional annotation were analyzed using different softwares. [Results] The final assembled genome size was approximately 55.2 Mb with an overall GC content of 53.5%. Further annotation analyses predicted 17094 protein-coding genes and 310 non-coding RNA genes. A large set of candidate genes involved in the production of pinoresinol, its glycoside derivatives and other secondary metabolites were identified. Orthology and phylogenetic analysis revealed that Phomopsis sp. XP-8 and 5 Ascomycota share 12635 orthologous genes and 5626 gene families. [Conclusion] Phomopsis sp. XP-8 possessed genomic basis for production of diverse secondary metabolites, including pinoresinol and its glycoside derivatives. This study provides basis for the further metabolic engineering of pinoresinol and its glucoside production.