Effect of zinc-bearing palygorskite on rumen bacterial diversity in vitro

Abstract:

[Objective] The aim of this experiment was to study the effects of zinc-bearing palygorskite (Zn-Pal) on rumen bacterial diversity in vitro.[Methods] We prepared Zn-Pal by the ion-exchange, and evaluated the compositions of bacterial communities in 60 samples based on 16S rDNA genes.[Results] We obtained a total of 1490959 effective sequences and 87662 OTUs. The bacterial diversity in the treatment IV group increased at 24 h, and the abundance of the treatment IV group increased at 48 h. Bacterial community composition analysis shows that the dominant phyla were Bacteroidetes, Firmicutes, Proteobacteria and Lentisphaerae. Compared with the control group, Firmicutes in treatment groups significantly increased (P<0.05) at 24 h and 48 h, whereas Bacteroidetes in the treatment IV group was decreased (P<0.05) at 48 h. At the genus level, the sequences could be assigned to 124 different genera. The content of Prevotella had no significant difference between the control and treatments. The relative abundance of Treponema in the treatment IV group was significantly higher at 48 h than in the control group (P<0.05), whereas the relative abundance of Victivallis and Pseudobutyrivibrio in treatment IV was lower.[Conclusion] Zn-Pal may affect rumen fluid bacterial diversity in dairy cows, and the degrees of this influence varied with the dose and time of Zn-Pal.