Adhesive inhibition comparison of Staphylococcus aureus to bovine mammary epithelial cells by antibodies against 3 different adhesins

Abstract:

[Objective] To compare the inhibition effects of antibodies against extracellular fibrinogen-binding protein (EfB) and fibronection binding protein (FnBPA) to clumping factor A (ClfA) of Staphylococcus aureus (SA) to bovine mammary epithelial cells (bMEC) by immunization method.[Methods] The antibodies were obtained by immunizing rabbits with the recombinant plasmids Efb or FnBPA in-combination with ClfA. The antibodies inhibition effects on adereing of 2 SA strains to bMEC were tested using plate counting method and observed by fluorescent staining of SA and bMEC, respectively.[Results] Our results indicated that the antibodies had different inhibition effects on adhesion of SA (GY278 and GY309). ClfA antibody showed the highest inhibition ability. FnBPA-A showed greater inhibition enhancement to ClfA than FnBPA-D.[Conclusion] The in-combination immunization of FnBPA-A, FnBPA-D and EfB with ClfA could affect the antibodies inhibition to various degrees and this result provided experiment basis for the development of bovine mastitis vaccine.