Analysis of germination-related proteins in Antrodia camphorata athroconidia by two-dimensional electrophoresis and mass spectrum

Abstract:

[Objective] Germination-related proteins in Antrodia camphorata athroconidia were analyzed by two-dimensional gel electrophoresis (2DE), mass spectrum, and real time fluorescent quantitative PCR (RT-qPCR).[Methods] We used 2DE to analyze total proteins of Antrodia camphorata arthroconidia after 0 h and 24 h of incubation. We identified differential proteins by PDQuest software and MALDI-TOF-MS. Then, we obtained germination-related proteins in Antrodia camphorata arthroconidia by matching the amino acid sequences of identified proteins to a local protein database. Finally, we used RT-qPCR to quantify relative expression levels of germination-related genes.[Results] A total of 32 differential expressed proteins, of which 25 up-regulated and 7 down-regulated, existed between non-germinated (0 h) and germinated (24 h) arthroconidia. Among these differential proteins, 24 proteins were successfully identified, and 10 proteins were involved in arthroconidial germination including GerO, Ubc1, Cat-1, Snf1, Cas2, SfaD, Chaperonin, Fad5, Tyrosine-P, and ChiA.[Conclusion] The results provide a theoretical basis for understanding of molecular mechanisms of athroconial germination of Antrodia camphorata.