Transcriptome analysis of recombinant Komagataella phaffii with methanol induced expression of phospholipase A2

Abstract:

[Objectives] In order to study the mechanism of heterologous protein expression in Komagataella phaffii, transcriptomics technology was used to study the profile of differentially expressed genes in the recombinant K. phaffii during the methanol-induced expression of phospholipase A₂.[Methods] We used RNA sequencing (RNA-seq) technology to analyze the differentially expressed genes in the recombinant K. phaffii expressing phospholipase A₂ between glycerol and methanol cultivation condition.[Results] In total 5225 transcripts were identified in the recombinant K. phaffii. Compared between glycerol and methanol cultivation condition, 857 genes were significant differentially expressed. According to KEGG (Kyoto Encyclopedia of Genes and Genomes) analysis, the differentially expressed genes were mostly related to ribosome composition, methanol metabolism, pentose phosphate pathway, citric acid cycle, glyoxylic acid cycle and protein processing process.[Conclusions] A global effect on the metabolism was observed when changing the carbon source from glycerol to methanol for the induction of heterologous protein. Our results provide rich information for further in-depth studies of the mechanism of protein expression in K. phaffii.