Abstract:Heredity and variation embody the beauty and mystery of life. In the process of continuous exploration, researchers have established genetics that is full of rigorous systematic and scientific methods. It is generally known that gene editing tools are indispensable in the study of genetics. CRISPR/Cas discovered in recent years as a new gene editing toolbox, has many characteristics, such as strong specificity and target ability, and become a hot spot of research and development. Here, we review the latest progress of CRISPR/Cas system including diversity, molecular mechanism and application.