Probiotic role of Lactobacillus plantarum Lp3 on hyperlipidemia rats

Abstract:

[Objective] In this study, we explored the probiotic effect of Lactobacillus plantarum Lp3 on fatty rats.[Methods] In vitro Lp3 with high hypocholesterolemic effects was isolated from Yak yogurt in Qinghai Tibet Plateau. We established a Sprague Dawley rat model of hyperlipidemia to determine the effect of the strain on blood lipids in experimental animals, and to study the effects on the intestinal flora, fecal moisture, cholesterol levels and bile acids of fecal, and the changes of cholesterol and triglycerides levels in the liver of SD rats. Thirty rats were assigned to three groups and fed either a normal or a high-cholesterol diet. The LAB treated group received the high-cholesterol diet supplemented with Lactobacillus plantarum Lp3.[Results] Lactobacillus plantarum Lp3 had no obvious side effects in rats and had a good cholesterol-lowering effect in high fat model rats. Lactobacillus plantarum Lp3 could significantly reduce serum total cholesterol, triglycerides and LDL-cholesterol levels in rats fed a high fat diet compared with the control group (P<0.05), but no difference was observed in high-density lipoprotein cholesterol. Compared with other two groups of rats, number of E. coli in feces of high fat fed rats group (HC) had obvious increase, the number of Bifidobacterium and Lactobacillus decreased significantly. But in high fat and LAB fed rats group (HL), the number of Lactobacillus in the feces was slightly higher than that in control group. The number of E. coli and Bifidobacteria were almost same as that in the control group. The results indicated that Lp3 could maintain the balance of intestinal flora and increase the moisture content in feces about 6.44% compared with HC group. Cholesterol and triglyceride in the liver tissue of the high fat fed rats group (HC) were significantly higher than high fat and LAB fed rats HL group (P<0.05). Lp3 could reduce lipid deposition in liver tissue. From histology of liver steatosis, we could also draw out the above conclusion. Cholesterol and bile acid contents in feces of HL group were higher than the HC group.[Conclusion] Lactobacillus plantarum Lp3 has probiotic potentials.