Analysis of salt tolerance of Lactobacillus plantarum FS5-5 based on iTRAQ technology

Abstract:

[Objective] Proteomic analysis of Lactobacillus plantarum to salt stress could provide a theoretical basis for salt-tolerant mechanism involved in lactic acid bacteria.[Methods] Lactobacillus plantarum FS5-5 was originally isolated from traditional home-made fermented soybean paste from Northeast China. Growth was observed under 0%, 6.0%, 7.0% and 8.0% (W/V) NaCl, and protein expression was analyzed using iTRAQ technology.[Results] We collected bacteria at 5, 10, 12 and 12 h of the mid-logarithmic growth phase for proteomic analysis. Based on iTRAQ results, a total of 1271 differently expressed proteins were identified under different salt stress. They were involved in carbohydrate metabolism, amino acid metabolism, lipid metabolism, nucleotide metabolism, stress proteins, transporters, phosphotransferase system, ribosome, and so on.[Conclusion] The ability of L. plantarum FS5-5 to grow in high concentration of NaCl is connected with energy synthesis proteins, stress proteins and compatible solutes transporters.