Effect of heavy metal Cu on Allogynogenetic Carassius auratus intestinal microorganism

Abstract:

[Objective] To study the effect of the heavy metal Cu on the bacterial composition and diversity in Allogynogenetic Carassius auratus intestinal.[Methods] Total DNA was extracted by DNA extraction kit. The 16S rRNA gene of intestinal microbial diversity was amplified by universal PCR primers. Then the clone library was built to analyze data.[Results] The main bacterial community in Allogynogenetic Carassius auratus intestinal contained Firmicutes, Proteobacteria and Bacteroidetes. At different concentrations of Cu, the contents of Firmicutes in Allogynogenetic Carassius auratus gut significantly decreased. The results of Rarefaction Curyiyuyiyuve, Venn and Diversity index showed that gut microbial diversity decreased obviously.[Conclusion] Cu caused the microbial diversity in Allogynogenetic Carassius auratus decreased. This result provides a basis for further studies on the health of Allogynogenetic Carassius auratus relevant to heavy metal Cu.