Diversity of camel rumen bacteria degrading nitrogen heterocyclic compounds

Abstract:

[Objective] As a typical desert animal, camel can eat pungent poisonous plants that are not eaten by other animals, and without affecting their normal physiological metabolism. Many studies found that phytotoxic substances in plants eaten by camel have similar chemical structure to that of pyridine, quinolone, indole and other heterocyclic compounds. However, few studies explored the biodiversity of bacteria degrading potentially heterocyclic compounds in camel rumen. [Methods] We used pyridine, quinolone and indole as the only carbon and nitrogen resources, and five generations of enrichment culture method to cultivate camel rumen bacteria, and used high throughput sequencing (Illumina Miseq) to sequence the total DNA of the five generations culture broth. [Results] Proteobacteria, Actinobacteria, Bacteroidetes, Planctomycetes and Firmicutes constitute the highest abundance of five categories of camel rumen bacteria degrading heterocyclic compounds. The dominant bacteria of degrading pyridine, quinolone, indole may belong to Sphingobacterium and Acinetobacter, Bacillus, Lysinibacillus and Sphingobacterium. [Conclusion] Camel rumen has heterocyclic compounds degrading bacteria.