Transfer of antimicrobial resistant genes of ESBL-producing Escherichia coli recovered from a wastewater treatment plant
CSTR:
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    [Objective] We explored the role of mobile plasmids in transferring antimicrobial resistant genes in extended-spectrum beta-lactamases (ESBLs)-producing Escherichia coli from a wastewater treatment plant. [Methods] Based on the conjugation experiments of ESBL-producing E. coli collected from wastewater, the disk diffusion assay and polymerase chain reaction (PCR) were used to determine the transfer of antimicrobial resistance. [Results] A total of 50 ESBL-producing E. coli were collected from 80 water samples (50/80, 62.5%), and successful conjugations were detected among 35 isolates (35/70, 70.0%). The results of PCR further showed that blaCTX-M and blaTEM genes were all capable of conjugation transfer, but the horizontal transfer of blaSHV gene was only detected in one isolate and resistance gene encoding fluoroquinolone was not capable of conjugation transfer in this study. [Conclusions] Plasmids carrying different antimicrobial resistant genes may exhibit different capacity of horizontal transfer. Importantly, the mobile plasmids played a very important role in the horizontal transfer of antimicrobial resistance genes of ESBL-producing E. coli.

    Reference
    Related
    Cited by
Get Citation

Qing Li, Hongna Zhang, Yuqing Liu, Jing Zhai, Weishan Chang. Transfer of antimicrobial resistant genes of ESBL-producing Escherichia coli recovered from a wastewater treatment plant. [J]. Acta Microbiologica Sinica, 2017, 57(5): 681-689

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:August 31,2016
  • Revised:December 01,2016
  • Adopted:
  • Online: May 02,2017
  • Published:
Article QR Code