Breeding and fermentation performance of a high-yield ε-poly-Llysine producing strain
CSTR:
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    [Objective] A high yield of ε-poly-L-lysine (ε-PL) producing strain was bred, and the effect of different carbon sources on fermentation performance was studied.[Methods] Genome shuffling and ribosome engineering were used to enhanced strain's productivity, and pH shock strategy was used to fermentation using different carbon sources.[Results] After four rounds of genome shuffling and ribosome engineering, we obtained a high yield mutant Streptomyces albulus GS114 with ε-PL productivity of 3.0 g/L, which was 1.7 folds than that of the initial strain. When we performed fed-batch fermentation using glucose and glycerol as carbon sources in a 5-L fermenter, ε-PL productions reached 43.4 and 45.7 g/L after 192 h fed-batch fermentations, which were increased by 11.0% and 14.9% than that of Streptomyces albulus M-Z18, respectively. Meanwhile, the dry cell weights decreased by 24.0% and 33.2%, and ε-PL yields increased by 34.2% and 30.7%, respectively.[Conclusion] Genome shuffling and ribosome engineering are effective to breed high yield strains.

    Reference
    Related
    Cited by
Get Citation

Gencheng Zheng, Liang Wang, Yang Gao, Jinxin Xiang, Xusheng Chen, Zhonggui Mao. Breeding and fermentation performance of a high-yield ε-poly-Llysine producing strain. [J]. Acta Microbiologica Sinica, 2016, 56(9): 1450-1458

Copy
Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:December 07,2015
  • Revised:February 22,2016
  • Adopted:
  • Online: September 01,2016
  • Published:
Article QR Code