Whole-genome sequencing and analysis of a adrostenedione yielding strains Mycobacterium neoaurum MN4

Abstract:

[Objective] Mycobaterium neoaurum MN4 is a substrate-resistant mutant strain with high-yield androstenedione. In order to further study MN4 strain substrate-resistant mechanism and androstenedione biosynthetic pathway, it is necessary to decipher the MN4 strain genome.[Methods] The genome was sequenced using highthroughput sequencing technology, and analyzed using relevant software for genome assembly, gene prediction and functional annotation, COG cluster analysis and secondary metabolite biosynthesis gene clusters prediction.[Results] The whole genome is assembled into 33 contigs, and the genome size is 5.39 Mb, GC content of 66.9% with encoding 4920 protein genes. The genome sequence was deposited in the GenBank database under the accession number JXYZ00000000.[Conclusion] This study is the first report of androstenedione producing strain Mycobacterium neoaurum MN4 genome sequence, and provides a theoretical basis for further heterologous expression of secondary metabolites on Mycobacterium neoaurum MN4.