Snf1/AMPK affects cell wall integrity through regulating the transcription of cell wall as sembly-related genesin Saccharomyces cerevisiae

Abstract:

[Objective] To study the role of Snf1/AMPK kinase in cell wall integrity in Saccharomyces cerevisiae JY102. [Methods] SNF1 deletion mutant of S. cerevisiae was created by homologous recombination. Congo red and Calcofluor white were applied to evaluate the cell wall integrity for the mutant strain. qRT-PCR was used to analyze the transcription of cell wall-related genes. [Results] The SNF1 disruption mutant was severely sensitive to Congo red and Calcofluor white, manifesting the impairment of cell wall integrity. The mutant exhibited apparently growth defect at high temperature. The results of qRT-PCR revealed down-regulated transcription of several genes involved in β-glucan biosynthesis. [Conclusion] The deletion of Snf1 impairs the cell wall integrity by reducing the transcription of β-glucan-related genes, suggesting a new role of Snf1 in the activation of cell wall synthesis.