Screening siderophore activity of four strains from alkaline environment

Abstract:

[Objectives] Siderophore is a low molecular iron chelate produced by microorganisms. It has broad application prospects in medicine, environmental restoration, health food and other fields. According to the literature survey so far, no siderophore was found from alkaline environment eukaryotes. Therefore, screening of fungi with high siderophore activity is of great significance. [Methods] By chromium azure S coloration, we screened 99 fungi isolated from Cheng Hai (an alkaline lakes in Yunnan province) and Datun alkaline tailings (Gejiu, Yunnan province). By spectrophotometric detection, we investigated the strain capacity of siderophore and type of siderophore. By solid phase extraction, we investigated the siderophore enrichment effect. Based on electron microscopy morphologic observation and ITS gene phylogenetic tree construction, we identified the strain. [Results] Strains FEDT-866, FEDT-145, FECH-998 and FECH-595 were siderophore high-yield ones. Except for strain FEDT-866, the siderophore active substances were suitable for solid phase extraction (SPE). Strains FEDT-866 and FECH-998 belong to Aspergillus and have higher similarity with Aspergillus tubigensis and A. nomius, respectively. Strains FECH-595, FEDT-145 belong to Penicillium and have higher similarity with P. svalbardense and P. chrysogenum. [Conclusion] We isolated and identified four fungi for possible siderophore production.