wzzE does not affect lipopolysaccharide phenotype of avian pathogenic Escherichia coli from clinical ducking

Abstract:

[Objective] wzz is involved in the synthesis of O antigen and plays a role in virulence in many gram-negative bacteria. However, the function of wzzE in avian pathogenic Escherichia coli (APEC) is unclear. The aim of this study is to elucidate the role of wzzE in the synthesis of LPS and virulence. [Methods] Mutant strain with wzzE deletation was constructed. Biological characteristics of the wild and mutant strains, such as LD₅₀, adherence and invasion to DF-1 cells, phenotype of LPS and endotoxin titer, were detected. [Results] There was no significant difference in growth, adherence and invasion to DF-1 cells, serotype and the endotoxin titer between the wide strain and the mutant. However, compared with the wild-type strain, the virulence of DE17ΔwzzE had dropped by 10 times. [Conclusion] wzzE is not involved in the synthesis of LPS in DE17, but participates in the pathogenic process of APEC. However, the mechanism of wzzE in APEC virulence needs to be studied in the future.