Semi-in vitro biosynthesis of cryptic lanthipeptide CLA 124 from Streptomyces clavuligerus
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Supported by the Project of Knowledge Innovation of Chinese Academy of Sciences (KSCX2-EW-Q-14,KSCX2-EW-J-6)

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    Abstract:

    Abstract:[Objective]To obtain the cryptic lanthipeptide from Streptomyces clavuligerus by semi-in vitro biosynthesis that is a novel method for mining lanthipeptides resource from Streptomyces.[Methods] The core peptide of cryptic lanthipeptide was modified in E.coli by nisin modification system,and purified by affinity chromatography and High Performance Liquid Chromatography (HPLC). After the leader peptide was removed,the core peptide was obtained and its dehydration and cyclic structure were analyzed by MALDI-TOF MS and tandem MS.[Results]A novel lanthipeptide named CLA 124 with 4-fold dehydration 2 thioether bridges and one disulfide bridge was produced.[Conclusion]Cryptic lanthipeptides from Streptomyces could be produced by semi-in vitro biosynthesis.

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Zheng Zhang, Li Zhang, Jie Zhang, Hongchu Ma, Shutao Sun, Jin Zhong. Semi-in vitro biosynthesis of cryptic lanthipeptide CLA 124 from Streptomyces clavuligerus. [J]. Acta Microbiologica Sinica, 2015, 55(11): 1402-1408

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History
  • Received:March 19,2015
  • Revised:April 23,2015
  • Adopted:
  • Online: November 03,2015
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