PFKFB3 regulates 11'-deoxyverticillin A (C42)-induced autophagy and apoptosis in HeLa cells

Abstract:

Abstract:［Objective］The aim is to reveal the role of PFKFB3 in 11'-deoxyverticillin A(C42)-induced autophagy and apoptosis.［Methods］Electron and fluorescence microscopy，immunoblotting，MTS assay，siRNA interference and real time PCR were used.［Results］C42 could induce multiple cell death in HeLa cells． Knockdown of either Beclin 1 or LC3，two important autophagic genes，increased both PARP-1 cleavage and cell viability loss． Although high dose of C42 triggered more cell viability loss，yet，it failed to augment autophagic flux． While PFKFB3 inhibitors attenuated C42-induced autophagy，the overexpression of PFKFB3 increased the induced autophagic flux.［Conclusion］PFKFB3 is involved in C42 induced-autophagy，which blunts the caspase-dependent apoptotic process.