Screening and antibacterial function of Bacillus amyloliquefaciens X030

Abstract:

Abstract:［Objective］We isolated 339 bacillus strains from 72 soil samples all over the country，then purified their antimicrobial compounds and studied the antibacterial activity，to enrich bacillus resources and explore their second metabolites.［Methods］A bacillus strain with strong antibacterial activity was selected by dilution plate and water bath heating from a soil sample from a peanut plantation in Henan Province; this strain was identified according to morphological observation，physiological and biochemical characteristics，and consequences of 16S rRNA homologous analysis． Antibacterial compound from the identified strain，Bacillus amyloliquefaciens X030，was separated and purified by acetone precipitation，Sephadex chromatography，C18 reverse phase column chromatography．Its molecular weight was analyzed by LC-MS/MS． The antibacterial activity was characterized by disc diffusion and plate two-way cultivation．［Results］Bacillus amyloliquefaciens was isolated that not only has antibacterial activity against Staphylococcus aureus，Candida albican and Saccharomycetes; but also against Pyriculariaoryzae，Chili pointed cell anthrax，Gloeosporium eriobotryae speg and Phytophthora parasitica．The compound was confirmed as polypeptide．［Conclusion］ Bacillus amyloliquefaciens X030 can produce a polypeptide that inhibits pathogenic bacteria and plant pathogenic fungi．