Purification， identification and characterization of an endoglucanase Egn20 from Fusarium sp.Q7-31T

Abstract:

Abstract:［Objective］The endoglucanase from Fusarium sp.Q7-31T was isolated，purified，identified and characterized to provide data for enzyme system of Fusarium sp.［Methods］Strain was cultured in liquid fermentation with oat strawas carbon source，the endoglucanase was purified by using Sephacry S-100 chromatography and DEAE-sepharose ionexchange column chromatography and the enzymatic properties were studied．The protein was identified using MADILTOF-TOF.［Results］ An endoglucanase was purified and named Egn20． The molecular weight was 55.37 kDa and isoelectric point (pI) was 7.44． Egn20 had optimal activity with carboxymethyl cellulose at 40 ℃ and pH6. 0，stabilized at 45 ℃ and pH5.0－7.0，activated by Fe2+，inhibited by Na+，Ca2+，Mg2+，Zn2+，K + and inactivated by Hg2+．The enzymatic properties and MADIL-TOF-TOF results suggested that Egn20 belongs to GH7 family.［Conclusion］ Our results may provide important data for the study of Fusarium sp．enzyme system.