Diversity of faecal bacteria in several mammals by 454 high-throughput sequencing

Abstract:

Abstract:［Objective］The aim of this study was to analyze the diversities and differences of several mammalian’s faecal bacteria，to understand the relationships between bacterium diversities and animals‘ evolutionary and animals’feeds.［Methods］ Genomic DNA of feces was extracted and amplified for the 16S rDNA V3 tags，and then the tags were sequenced by 454 sequencing．QIIME were used to analyze faecal bacterial diversities.［Results］Faecal bacteria of all animals were dominated by Firmicutes，Bacteroidetes and Proteobacteria． Bacterial diversities of Hylobates hoolock，Pan troglodytes and Rhinopithecus roxellanae were the highest，followed by Panthera tigris altaica，Ailuropoda melanoleuca and Ursus thibetanus were the lowest through α diversity analysis．The constituents of faecal bacteria among Hylobates hoolock，Pan troglodytes and Ｒhinopithecus roxellanae were similar．The constituents of faecal bacteria among Ailuropoda melanoleuca，Ursus thibetanu，and Panthera tigris altaica were similar． Mainly for containning Fusobacteria，the faecal bacterial of Panthera tigris altaica differed from the other two carnivore animals through β diversity analysis.［Conclusion］ The dominating faecal bacteria were obvious，the bacteria similarities of the two repetitions were the highest． The diversities of each animal were different and higher in the primates． Both evolution and food were related to faecal bacteria． This study provided some references for exploring the new microorganism and further research of faecal bacteria．